Fluoro-Gold：Fluorochrome公司少見授權(quán)代理，現(xiàn)貨促銷！

時間：2016-01-11作者：深圳市豪地華拓生物科技有限公司瀏覽：156

	中國一授權(quán)總代


	
美國 Fluorochorome 公司于1985 年成立于美國科羅拉多州丹佛市，F(xiàn)luorochorome 一直致力于熒光染料的研究與開發(fā)，其**產(chǎn)品Fluoro-Gold（熒光金）自 1985 年來在**范圍內(nèi)被廣泛應(yīng)用，并有大量的參考文獻。同時，F(xiàn)luorochrome公司特別開發(fā)了高靈敏度的 Fluoro-Gold Antibody 、以及Fluoro-Ruby（紅色熒光金）等產(chǎn)品，F(xiàn)luorochrome 公司堅 持以優(yōu)質(zhì)的產(chǎn)品和熱情的服務(wù)贏得了良好的**度和口碑。
	
		公司信息
	
    品牌商名稱：Fluorochrome.LLC 
    聯(lián)系電話：(303) 394-1000 
    聯(lián)系地址：1801 Williams Street, Suite 100 Denver, Colorado 80218 USA
    公司官網(wǎng)
	
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丁香通一代理認證鏈接/brand/ff80808151482b4d015157247a2467eb/brand.htm

拒絕假冒偽劣產(chǎn)品，維護客戶權(quán)益和品牌形象！

熒光金(Fluoro-Gold)是Fluorochrome 公司經(jīng)過多年研究開發(fā)出來的**產(chǎn)品。熒光金(Fluoro-Gold)在化學(xué)結(jié)構(gòu)上有區(qū)別于普通的羥脒替，從而物理性質(zhì)也截然不同。近30年來，F(xiàn)luoro-Gold占據(jù)**市場，得到廣大科研人員的一致認可。
假冒偽劣產(chǎn)品特點：供應(yīng)公司聲稱自己為品牌的一級授權(quán)代理，但均無授權(quán)證明；只宣傳宣傳推廣品牌，沒有詳細產(chǎn)品信息，并在其他正規(guī)網(wǎng)站均無授權(quán)認證信息。例如單做品牌的百度推廣，代理信息無法證明的；替代產(chǎn)品所用普通的熒光染料價格非常低廉，以假亂真有豐富的利潤可圖，包裝簡陋，均**市場均價，質(zhì)量差，效果存在較大差異，導(dǎo)致老師實驗受到影響 而重新訂購。 客戶和經(jīng)銷商朋友訂購時完全可要求提供Fluorochrome公司出貨單及國際快遞單號。務(wù)必確認產(chǎn)品為正規(guī)來源。

近期收到一些水貨及假貨的投訴，有的導(dǎo)致老師實驗受到嚴重影響，我們和Fluorochrome公司特此聲明：
深圳市豪地華拓生物科技有限公司為 Fluorochrome公司在中國大陸及港澳地區(qū)的*少見授權(quán)代理。我們可以提供完善的技術(shù)支持和售后**。 我公司2005年開始代理和推廣 Fluorochrome公司產(chǎn)品，其中文名稱“熒光金”源自廣州華拓公司，其貨號“FC10001、FC20001…“為華拓公司自編貨號：我們的價格優(yōu)勢-Fluorochrome**國內(nèi)較低價。

華拓生物公司聯(lián)系方式： e-mail:order@    Telephone:（86）0755-26055215
Fluorochrome公司直接聯(lián)系方式：e-mail: info@   Telephone:(303) 394-1000   Fax: (303) 321-1119    
客戶如訂購到偽劣產(chǎn)品請保留好訂購合同，發(fā)票，收貨單或快遞單號，并及時和我們聯(lián)系。
  


	


	                                         


	Fig.1 Amygdala cells (40X) after Fluoro-Gold injection in the PVN.  Antibody is at 1/50,000


	Fig.2 High magnification view of Fluoro-Ruby labeled axons, terminals and vascular pericytes as seen in the rat striatum following tracerinjection into the contralateral substantia nigra. 


	
	
		
			
				熒光金
			
			
				Fluoro-Gold
			
			
				20mg
			
			
				Fluorochrome
			
		
		
			
				熒光金
			
			
				Fluoro-Gold
			
			
				50mg
			
			
				Fluorochrome
			
		
		
			
				熒光金
			
			
				Fluoro-Gold
			
			
				100mg
			
			
				Fluorochrome
			
		
		
			
				熒光金
			
			
				Fluoro-Gold
			
			
				150mg
			
			
				Fluorochrome
			
		
		
			
				熒光金
			
			
				Fluoro-Gold
			
			
				200mg
			
			
				Fluorochrome
			
		
		
			
				熒光金抗體
			
			
				Antibody to Fluoro-Gold
			
			
				0.1ml×1
			
			
				Fluorochrome
			
		
		
			
				熒光金抗體
			
			
				Antibody to Fluoro-Gold
			
			
				0.1ml×2
			
			
				Fluorochrome
			
		
		
			
				熒光金抗體
			
			
				Antibody to Fluoro-Gold
			
			
				0.1ml×3
			
			
				Fluorochrome
			
		
		
			
				紅色熒光金
			
			
				Fluoro-Ruby
			
			
				30mg
			
			
				Fluorochrome
			
		
	



	
		熒光金說明書
	
	
		
FLUOROCHROME,LLC
1801 Williams Street, Suite 100
Denver, Colorado 80218 USA
Telephone:(303) 394-1000                                                                e-mail: info@
Fax: (303) 321-1119                                                                        website: 
	
	
		
	
	
		
			
				
					
						Fluoro-Gold Protocol and Use Guide
					
					
						Main Protocol
1. Background
The use of Fluoro-Gold is essentially the same as other fluorescent tracers. The main difference is that Fluoro-Gold is more flexible in terms of post-injection survival times, concentration range, tissue treatment and compatibility with other histochemical techniques.

2. Storage and Shelf Life
Dry Fluoro-Gold should be kept in a light tight closed container at 4 degrees Celsius. Stored properly, Fluorogold should have a shelf life exceeding one year. The dye in solution should also be kept in a light tight closed container at 4 degrees Celsius and should remain stable for at least six months.

3. Vehicle
Fluoro-Gold can be dissolved in distilled water or 0.9% saline, or utilized as a suspension
in 0.2M neutral phosphate buffer.

4. Dye Concentration
Fluoro-Gold has been successfully used at concentrations ranging from 1-10%. Initially, a 4% concentration is advised. If undesirable necrosis occurs at the injection site, or labeling is too intense, reduce the concentration to a 2% solution. If you need to use more precise measurements, the molecular weight of Fluoro-Gold is 532.6 daltons.

5. Dye Administration
					
					
						
							A. Pressure Injection - This is probably the most frequently used mode of application. Volumes injected range from .05-1 μl, typically .1-.2 μl.
						
						
							B. Iontophoresis - Discrete, small injection sites result from 4-10 second pulsed iontophoretic (+5 to +10ua/10min) application.
						
						
							C. Crystal - A crystal of the tracer can be administered from the tip of a micro-pipette.
						
					
					
						6. Post-0perative Survival Period
Good retrograde labeling has been observed with periods ranging from two days to two months. Survival periods of three to five days are typical. Long survival periods enhance filling of distal processes without diffusion of the dye from the cell.

7. Fixation
Almost any fixative, or no fixative, can be used, Phosphate neutral buffered saline containing 4% formaldehyde is frequently employed. Fixatives containing high concentrations of heavy metals (e.g. osmium, mercury) will quench the fluorescence, while high concentrations (over 1%) of glutaraldehyde may increase background fluorescence

8. Histochemical Processing
Tissue containing Fluoro-Gold may be processed according to virtually any common histological technique. This includes cryostat sections of unfixed tissue (10 μm), frozen sections of fixed tissue (20 μm), and thin sections cut from tissue imbedded in either plastic (.2-4 μm) or paraffin (3-10 μm). Frozen sections of fixed tissue are most frequently used.

9. Combined Methods
At this point of processing, sections may be further processed for a second marker such as autoradiography, HRP histochemistry, immunocytochemistry, a second fluorescent tracer, fluorescent counterstain, etc.

10. Mounting, Clearing and Coverslipping
Sections are typically mounted on gelatin-coated slides, air-dried, immersed in xylene, and coverslipped with nonfluorescent DPX plastic mounting media. Sections may be dehydrated with graded alcohols, unless this is not compatible with a second tracer. If Fluoro-Gold is to be combined with fluorescence immunocytochemistry, then sections are air-dried and directly coverslipped with neutral buffered glycerine (1:2). 

11. Examination and Photography
Fluoro-Gold can be visualized with a fluorescence microscope using a wide band ultraviolet excitation filter. A gold color is emitted when tissue has been processed with neutral pH buffer, whereas a blue color is emitted when tissue is processed with acidic (e.g. pH 3.3) pH buffer. It can be photographed digitally or with film (use Ektachrome 200-400 ASA film for color prints and comparable speed film for black and white prints, for example Tri-X). Most exposure times range from 10-60 second exposures, depending on the objective magnification and the intensity of the label. Thirty (30) second exposures are about average. Multiple exposures may be exploited to simultaneously visualize Fluoro-Gold and another tracer. Thus, UV would be combined with bright field illumination to simultaneously locate Fluoro-Gold with HRP or silver grains in autoradiography. Similarly, blue light excitation can be combined to also visualize the green emission color of FITC, while green excitation light may be used to simultaneously observe the red emission color of propidium iodide, or ethidium bromide (a fluorescent counterstain).

Additional Information Concerning the Use of Fluoro-Gold
Vehicle
For pressure injections through a microsyringe or micropipette, Fluoro-Gold should be dissolved in distilled water or .9% saline. Fluoro-Gold may also be utilized as a suspension in .2M neutral phosphate buffer, however, the suspended particles may clog a fine micropipette tip so distilled water or .9% saline is the preferred vehicle. For iontophoresis, a 1% Fluoro-Gold solution is made up in .1M acetate buffer (pH=3.3). Well-cleaned (95% ETOH, water) glass micropipettes should have tips of 10-20 μm. Optimal iontophoresis parameters are +1 to +5u amps delivered with pulsed current (4-10 seconds on, 4-10 seconds off) over a 10-20 minute period. 

Injection Sites
Virtually any central or peripheral nervous system structure can be injected with Fluoro-Gold for analysis of retrograde transport. In the peripheral nervous system, ganglia and peripheral targets can be studied. For studies of peripheral nerve, the nerve should be cut or damaged and either dipped in, or injected with, aq 5% solution of Fluoro-Gold. Since Fluoro-Gold is not significantly taken up by intact fibers of passage, the fibers must be cut or severely damaged for uptake of the dye to occur. 

Transport and Survival Time
Fluoro-Gold is used as a retrograde axonal tracer, although orthograde axonal transport does occur. The survival time should be varied (especially to very short survival times of 12 hours - 2 days) to maximize orthograde transport in the specific neuronal system under study. For retrograde transport, the survival times should be varied from 4 days to 14 days. Seven to 10 days works for most systems, although long pathways (e.g., spinal cord to brainstem) and pathways in large mammals (e.g., cats, monkeys) may require longer survival times (e.g., 14 days). In addition, since Fluoro-Gold remains fast within retrogradely labeled neurons, survival times of several months will also produce excellent results. For iontophoresis, a 2-5 day survival time is recommended. It is estimated that transport occurs at about 2 cm per day for mammals; it is slower for cold-blooded animals.

Tissue Processing
Tissue processing is covered in detail in the use guide and in the original publication (Schmued and Fallon, 1986, Brain Research 377:147-154). Since Fluoro-Gold is stable in many solvents and remains fast within retrogradely labeled neurons, it's use is compatible with many histochemical techniques. It can be used with other retrograde tracers, immunofluorescence, ** and ABC immunocytochemistry, HRP histochemistry, autoradiography, counterstains (ethidium bromide is the preferred fluorescent counterstain), paraffin embedding and plastic embedding. However, if tissue is unfixed, additional processing of tissue in aqueous solutions for over an hour or two will result in loss of Fluoro-Gold fluorescence from labeled neurons. Fluoro-Gold may be useful in electron microscopy. Fluoro-Gold can be used in a brain which has been sectioned and transferred to phosphate buffer. Sections are typically mounted on gelatin-coated slides, air dried, immersed in xylene and coverslipped with DPX plastic mounting media (FLUKA Chemical Corp., 255 Oser Avenue, Hauppauge, New York, 11788, Catalog #44581). Tissue may also be viewed on slides without further processing, can be run through graded alcohols for dehydration, or, for immunocytochemistry, the sections can be air dried and directly coverslipped with neutral buffered glycerine (1:2). 

Examination and Photography
Fluoro-Gold is visualized with a fluorescence microscope using a wide band ultraviolet (UV) excitation filter. Use the same filter pack you would for other fluorescent retrograde tracers excited under wide band UV (e.g., True Blue, Fast Blue, Nuclear Yellow), such as the Leitz Ploem filter system A (Wide Band UV, Excitation filter BP 340-380), Mirror RKP 400, Barrier Filter LP 430). Objectives should be made especially for fluorescence microscopy (such as that made by Zeiss) glycerine, or water. Since plastic does absorb UV light, it is not advised to view through plastic petri dishes, etc. Recommended films are T-Max (Kodak, black & white) and Ektachrome 200 (Kodak, color slides). Exposure times usually vary from 20 seconds to 1.5 minutes. 

Chemical Analysis
					
				
			
			
				
					
						
							
								
									Quality
								
								
									Expected Result
								
								
									Actual Result
								
							
							
								
									Appearance
								
								
									A golden-yellow, hygroscopic, crystalline powder
								
								
									A bright-yellow powder
								
							
							
								
									Odor
								
								
									None
								
								
									None
								
							
							
								
									Solution
								
								
									20 ml of a 5% w/v aqueous solution should be clean, clear and almost free from suspended matter, and should have not more than a very slight odor
								
								
									Passes Test
								
							
							
								
									pH of a 1% Solution
								
								
									Between 4.0 and 5.5 at 25 degrees Celsius
								
								
									4.6
								
							
							
								
									Spectral characteristics
								
								
									The spectral characteristics of Fluoro-Gold vary with pH
								
								
									
										A 0.1% solution in distilled water has a pH of 4.5 and excitation peak of 414 nm and emission peak of 541 nm
									
									
										Fluoro-Gold bound to membranes at a physiological pH of 7.4 has an excitation band of 350 to 395 nm and an emission band of 530 to 600 nm
									
								
							
							
								
									Chloride
								
								
									Not more than 0.035%
								
								
									0.017%
								
							
							
								
									Sulfate
								
								
									Not more than 0.1%
								
								
									Less than 0.05%
								
							
							
								
									Sulfated ash
								
								
									Not more than 0.1%
								
								
									Negligible
								
							
							
								
									Heavy metals
								
								
									Not more than 10 p.p.m
								
								
									Less than 10 p.p.m
								
							
							
								
									Selenium
								
								
									Not more than 30 p.p.m
								
								
									Less than 10 p.p.m.
								
							
							
								
									Loss on drying
								
								
									Not more than 1.0% after 3 hours in vacuo at 60 degrees Celsius
								
								
									0.1%
								
							
							
								
									Assay
								
								
									Between 95.0 and 105.0% calculated with reference to the dried material
								
								
									99.2%
								
							
						
					
					
						Notice: The original and only true Fluoro-Gold (Fluorogold) is produced by Fluorochrome, LLC and marketed by Fluorochrome, LLC and Histo-Chem Inc.
					
					
						Fluoro-Gold (Fluorogold) is an exclusive product of Fluorochrome, LLC. It has been sold by Fluorochrome and widely used since 1985. Other companies are marketing a product they claim  is the same as or equivalent to Fluoro-Gold. In fact, the chemical structures of these compounds seem to be different from Fluoro-Gold. Certain physical properties of the compounds may be very different.

*CAUTION: Fluoro-Gold, Antibody to Fluoro-Gold and Fluoro-Ruby are for investigational use only in laboratory research animals or for tests in vitro. NOT FOR USE IN HUMANS. These drugs should be used only by persons regularly engaged in conducting neuroanatomical studies and tests in vitro or in animals used only for laboratory research.
					
				
			
		
	


	深圳市豪地華拓生物


深圳市豪地華拓生物科技有限公司專注于抗體等

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• 熒光金，F(xiàn)luoro-Gold，F(xiàn)luorochrome公司產(chǎn)品促銷

  Fluorochrome公司熒光金Fluoro-Gold促銷！ Fluorochrome?公司的熒光金通過**認證，其Fluoro-Gold為**少見供應(yīng)。? 較近，國內(nèi)市場上出現(xiàn)假冒Fluorochrome的偽劣產(chǎn)品，導(dǎo)致老師的實驗受到很大影響。 偽劣假冒產(chǎn)品特點： 1，供應(yīng)公司聲稱自己為國外很多品牌的一級代理，但均無授權(quán)證明的。 2，供應(yīng)產(chǎn)品信息不全，宣傳推廣只宣傳國外某

• Fluoro-Gold：Fluorochrome公司少見授權(quán)代理，現(xiàn)貨促銷！

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